Gfp Fluorescence Spectrum
Many marine organisms have similar green fluorescent proteins but gfp refers to a protein which was originally isolated from this particular jellyfish.
Gfp fluorescence spectrum. The differences between the two include the principles that guide the work the procedures employed and the main objectives. Gfp and yfp are two very different fluorescent proteins derived from aequorea victoria a jellyfish. Visser 12 and olaf j. The fluorophore absorbs light energy of a specific wavelength and re emits light at a longer wavelength.
We now have jellyfish proteins that span an 80 nanometer portion visible spectrum from deep blue to yellow green providing a wide choice of genetically encoded markers for studies in cell biology. Many fluorescence spectrometers detect intensity in cps counts per second but the measured intensity in cps will not necessarily be the same from instrument to instrument. Rolinski 2 1 laboratory of biochemistry microspectroscopy centre wageningen university po. University of cyprus biomedical imaging and applied opticsbiomedical imaging and applied optics fluorescence spectroscopyfluorescence spectroscopy.
Basic photophysics antonie jwg. Wednesday february 11 2015. Biolegends fluorescence spectra analyzer is useful for the analysis of excitation and emission spectra of commonly used fluorochromes for flow cytometry. It is useful to know the excitation and emission spectra of fluorophores to help in selecting lights and filters for fluorescence.
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